Paul Kulesa, PhD
Title | Doctoral Research Faculty, Development and Behavioral Health |
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Institution | Children's Mercy Kansas City |
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Department | Developmental and Behavioral Health |
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Address | 2401 Gillham Rd Kansas City MO 64108
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vCard | Download vCard |
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Title | Investigator, Children's Mercy Research Institute |
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Institution | Children's Mercy Kansas City |
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Department | Pediatrics |
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Title | Professor, Department of Pediatrics UMKC SOM |
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Institution | University of Missouri-Kansas City |
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Department | Pediatrics |
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Biography University of Washington, Seattle, WA | Ph.D. | 1995 | Applied Mathematics | University of Southern California, Los Angeles, CA | MS | 1990 | Applied Mathematics | University of Notre Dame, Notre Dame, IN | BS | 1984 | Aerospace Engineering |
Overview Dr. Kulesa is a quantitative developmental biologist. His expertise lies at the intersection between biology and mathematics. He collects multi-scale data and constructs models to explain complex biological phenomena such as collective cell migration —a process essential to development of the nervous system and disease. His research focuses on neural crest cell development and how mistakes in migration and patterning lead to human birth defects and disease. Neural crest cells are highly migratory embryonic cells in vertebrates —animals with backbones.
Before joining Children’s Mercy, Dr. Kulesa was the founding Director of Imaging at the Stowers Institute for Medical Research. During his almost two decades at the Stowers Institute, he established an internationally recognized program in neural crest cell biology. While there, he trained students in techniques to study cell migration during vertebrate development, using neural crest and neural crest-derived cancer models. Following his work at the Stowers Institute, he was the Berthiaume Family Professor of Neuroscience at the University of Notre Dame
Bibliography
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Morrison JA, Pushel I, McLennan R, McKinney MC, Gogol MM, Scott A, Krumlauf R, Kulesa PM. Comparative Analysis of Neural Crest Development in the Chick and Mouse. bioRxiv. 2024 Nov 06. PMID: 39574664.
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Kasemeier-Kulesa JC, Martina Perez S, Baker RE, Kulesa PM. Identification of Neural Crest and Neural Crest-Derived Cancer Cell Invasion and Migration Genes Using High-throughput Screening and Deep Attention Networks. bioRxiv. 2024 Mar 10. PMID: 38496683.
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Martinson WD, McLennan R, Teddy JM, McKinney MC, Davidson LA, Baker RE, Byrne HM, Kulesa PM, Maini PK. Dynamic fibronectin assembly and remodeling by leader neural crest cells prevents jamming in collective cell migration. Elife. 2023 04 19; 12. PMID: 37073859.
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Kasemeier-Kulesa JC, Morrison JA, McKinney S, Li H, Gogol M, Hall K, Chen S, Wang Y, Perera A, McLennan R, Kulesa PM. Cell-type profiling of the sympathetic nervous system using spatial transcriptomics and spatial mapping of mRNA. Dev Dyn. 2023 08; 252(8):1130-1142. PMID: 36840366.
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McLennan R, Giniunaite R, Hildebrand K, Teddy JM, Kasemeier-Kulesa JC, Bolanos L, Baker RE, Maini PK, Kulesa PM. Colec12 and Trail signaling confine cranial neural crest cell trajectories and promote collective cell migration. Dev Dyn. 2023 05; 252(5):629-646. PMID: 36692868.
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Morrison JA, McLennan R, Teddy JM, Scott AR, Kasemeier-Kulesa JC, Gogol MM, Kulesa PM. Single-cell reconstruction with spatial context of migrating neural crest cells and their microenvironments during vertebrate head and neck formation. Development. 2021 11 15; 148(22). PMID: 35020873.
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Kasemeier-Kulesa JC, Spengler JA, Muolo CE, Morrison JA, Woolley TE, Schnell S, Kulesa PM. The embryonic trunk neural crest microenvironment regulates the plasticity and invasion of human neuroblastoma via TrkB signaling. Dev Biol. 2021 12; 480:78-90. PMID: 34416224.
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Kulesa PM, Kasemeier-Kulesa JC, Morrison JA, McLennan R, McKinney MC, Bailey C. Modelling Cell Invasion: A Review of What JD Murray and the Embryo Can Teach Us. Bull Math Biol. 2021 02 17; 83(4):26. PMID: 33594536.
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McKinney MC, Kulesa PM. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo. Methods Mol Biol. 2021; 2179:107-114. PMID: 32939716.
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McKinney MC, McLennan R, Giniunaite R, Baker RE, Maini PK, Othmer HG, Kulesa PM. Visualizing mesoderm and neural crest cell dynamics during chick head morphogenesis. Dev Biol. 2020 05 15; 461(2):184-196. PMID: 32084354.
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McLennan R, McKinney MC, Teddy JM, Morrison JA, Kasemeier-Kulesa JC, Ridenour DA, Manthe CA, Giniunaite R, Robinson M, Baker RE, Maini PK, Kulesa PM. Neural crest cells bulldoze through the microenvironment using Aquaporin 1 to stabilize filopodia. Development. 2020 01 09; 147(1). PMID: 31826865.
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Giniunaite R, McLennan R, McKinney MC, Baker RE, Kulesa PM, Maini PK. An interdisciplinary approach to investigate collective cell migration in neural crest. Dev Dyn. 2020 03; 249(3):270-280. PMID: 31622517.
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Giniunaite R, Baker RE, Kulesa PM, Maini PK. Modelling collective cell migration: neural crest as a model paradigm. J Math Biol. 2020 01; 80(1-2):481-504. PMID: 31587096.
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Moustakas-Verho JE, McLennan R, Spengler J, Kulesa PM, Cebra-Thomas JA. Manipulation of Developmental Function in Turtles with Notes on Alligators. Methods Mol Biol. 2019; 1920:247-263. PMID: 30737695.
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McLennan R, Kulesa PM. In Ovo Electroporation of Plasmid DNA and Morpholinos into Specific Tissues During Early Embryogenesis. Methods Mol Biol. 2019; 1976:71-82. PMID: 30977066.
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Moustakas-Verho JE, McLennan R, Spengler J, Kulesa PM, Cebra-Thomas JA. Correction to: Manipulation of Developmental Function in Turtles with Notes on Alligators. Methods Mol Biol. 2019; 1920:C1. PMID: 31290130.
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Dyson L, Holmes A, Li A, Kulesa PM. A chemotactic model of trunk neural crest cell migration. Genesis. 2018 09; 56(9):e23239. PMID: 30133140.
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Kasemeier-Kulesa JC, Schnell S, Woolley T, Spengler JA, Morrison JA, McKinney MC, Pushel I, Wolfe LA, Kulesa PM. Predicting neuroblastoma using developmental signals and a logic-based model. Biophys Chem. 2018 07; 238:30-38. PMID: 29734136.
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Kasemeier-Kulesa JC, Kulesa PM. The convergent roles of CD271/p75 in neural crest-derived melanoma plasticity. Dev Biol. 2018 12 01; 444 Suppl 1:S352-S355. PMID: 29660313.
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Kasemeier-Kulesa JC, Romine MH, Morrison JA, Bailey CM, Welch DR, Kulesa PM. NGF reprograms metastatic melanoma to a bipotent glial-melanocyte neural crest-like precursor. Biol Open. 2018 Jan 11; 7(1). PMID: 29175861.
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Morrison JA, McLennan R, Wolfe LA, Gogol MM, Meier S, McKinney MC, Teddy JM, Holmes L, Semerad CL, Box AC, Li H, Hall KE, Perera AG, Kulesa PM. Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions. Elife. 2017 12 04; 6. PMID: 29199959.
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McLennan R, Bailey CM, Schumacher LJ, Teddy JM, Morrison JA, Kasemeier-Kulesa JC, Wolfe LA, Gogol MM, Baker RE, Maini PK, Kulesa PM. DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion. J Cell Biol. 2017 10 02; 216(10):3339-3354. PMID: 28811280.
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Morrison JA, McKinney MC, Kulesa PM. Resolving in vivo gene expression during collective cell migration using an integrated RNAscope, immunohistochemistry and tissue clearing method. Mech Dev. 2017 12; 148:100-106. PMID: 28633909.
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McKinney MC, McLennan R, Kulesa PM. Angiopoietin 2 signaling plays a critical role in neural crest cell migration. BMC Biol. 2016 12 15; 14(1):111. PMID: 27978830.
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Schumacher LJ, Kulesa PM, McLennan R, Baker RE, Maini PK. Multidisciplinary approaches to understanding collective cell migration in developmental biology. Open Biol. 2016 06; 6(6). PMID: 27278647.
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Kasemeier-Kulesa JC, Morrison JA, Lefcort F, Kulesa PM. TrkB/BDNF signalling patterns the sympathetic nervous system. Nat Commun. 2015 Sep 25; 6:8281. PMID: 26404565.
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McLennan R, Schumacher LJ, Morrison JA, Teddy JM, Ridenour DA, Box AC, Semerad CL, Li H, McDowell W, Kay D, Maini PK, Baker RE, Kulesa PM. VEGF signals induce trailblazer cell identity that drives neural crest migration. Dev Biol. 2015 Nov 01; 407(1):12-25. PMID: 26278036.
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Morrison JA, Box AC, McKinney MC, McLennan R, Kulesa PM. Quantitative single cell gene expression profiling in the avian embryo. Dev Dyn. 2015 Jun; 244(6):774-84. PMID: 25809747.
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McLennan R, Schumacher LJ, Morrison JA, Teddy JM, Ridenour DA, Box AC, Semerad CL, Li H, McDowell W, Kay D, Maini PK, Baker RE, Kulesa PM. Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front. Development. 2015 Jun 01; 142(11):2014-25. PMID: 25977364.
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Kulesa PM, McLennan R. Neural crest migration: trailblazing ahead. F1000Prime Rep. 2015; 7:02. PMID: 25705385.
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Bailey CM, Kulesa PM. Dynamic interactions between cancer cells and the embryonic microenvironment regulate cell invasion and reveal EphB6 as a metastasis suppressor. Mol Cancer Res. 2014 Sep; 12(9):1303-13. PMID: 24836890.
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Ridenour DA, McLennan R, Teddy JM, Semerad CL, Haug JS, Kulesa PM. The neural crest cell cycle is related to phases of migration in the head. Development. 2014 Mar; 141(5):1095-103. PMID: 24550117.
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Kulesa PM, Morrison JA, Bailey CM. The neural crest and cancer: a developmental spin on melanoma. Cells Tissues Organs. 2013; 198(1):12-21. PMID: 23774755.
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Wynn ML, Rupp P, Trainor PA, Schnell S, Kulesa PM. Follow-the-leader cell migration requires biased cell-cell contact and local microenvironmental signals. Phys Biol. 2013 Jun; 10(3):035003. PMID: 23735560.
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Kulesa PM, McKinney MC, McLennan R. Developmental imaging: the avian embryo hatches to the challenge. Birth Defects Res C Embryo Today. 2013 Jun; 99(2):121-33. PMID: 23897596.
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McKinney MC, Fukatsu K, Morrison J, McLennan R, Bronner ME, Kulesa PM. Evidence for dynamic rearrangements but lack of fate or position restrictions in premigratory avian trunk neural crest. Development. 2013 Feb; 140(4):820-30. PMID: 23318636.
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Morrison JA, Bailey CM, Kulesa PM. Gene profiling in the avian embryo using laser capture microdissection and RT-qPCR. Cold Spring Harb Protoc. 2012 Dec 01; 2012(12). PMID: 23209136.
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Bailey CM, Morrison JA, Kulesa PM. Melanoma revives an embryonic migration program to promote plasticity and invasion. Pigment Cell Melanoma Res. 2012 Sep; 25(5):573-83. PMID: 22681858.
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McLennan R, Dyson L, Prather KW, Morrison JA, Baker RE, Maini PK, Kulesa PM. Multiscale mechanisms of cell migration during development: theory and experiment. Development. 2012 Aug; 139(16):2935-44. PMID: 22764050.
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Ridenour DA, McKinney MC, Bailey CM, Kulesa PM. CycleTrak: a novel system for the semi-automated analysis of cell cycle dynamics. Dev Biol. 2012 May 01; 365(1):189-95. PMID: 22387844.
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Wynn ML, Kulesa PM, Schnell S. Computational modelling of cell chain migration reveals mechanisms that sustain follow-the-leader behaviour. J R Soc Interface. 2012 Jul 07; 9(72):1576-88. PMID: 22219399.
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McKinney MC, Kulesa PM. In vivo calcium dynamics during neural crest cell migration and patterning using GCaMP3. Dev Biol. 2011 Oct 15; 358(2):309-17. PMID: 21864525.
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Kulesa PM, Dickinson ME, Hadjantonakis AK. Highlights of the special imaging issue. Genesis. 2011 Jul; 49(7):479-83. PMID: 21739566.
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McKinney MC, Stark DA, Teddy J, Kulesa PM. Neural crest cell communication involves an exchange of cytoplasmic material through cellular bridges revealed by photoconversion of KikGR. Dev Dyn. 2011 Jun; 240(6):1391-401. PMID: 21472890.
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Kasemeier-Kulesa JC, McLennan R, Romine MH, Kulesa PM, Lefcort F. CXCR4 controls ventral migration of sympathetic precursor cells. J Neurosci. 2010 Sep 29; 30(39):13078-88. PMID: 20881125.
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Kulesa PM, Teddy JM, Smith M, Alexander R, Cooper CH, Lansford R, McLennan R. Multispectral fingerprinting for improved in vivo cell dynamics analysis. BMC Dev Biol. 2010 Sep 24; 10:101. PMID: 20868502.
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McLennan R, Kulesa PM. Neuropilin-1 interacts with the second branchial arch microenvironment to mediate chick neural crest cell dynamics. Dev Dyn. 2010 Jun; 239(6):1664-73. PMID: 20503363.
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Steen J, Morrison JA, Kulesa PM. Multi-position photoactivation and multi-time acquisition for large-scale cell tracing in avian embryos. Cold Spring Harb Protoc. 2010 Jun; 2010(6):pdb.prot5447. PMID: 20516185.
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Kulesa PM, Bailey CM, Cooper C, Fraser SE. In ovo live imaging of avian embryos. Cold Spring Harb Protoc. 2010 Jun; 2010(6):pdb.prot5446. PMID: 20516184.
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Kulesa PM, Gammill LS. Neural crest migration: patterns, phases and signals. Dev Biol. 2010 Aug 15; 344(2):566-8. PMID: 20478296.
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Kulesa PM, Bailey CM, Kasemeier-Kulesa JC, McLennan R. Cranial neural crest migration: new rules for an old road. Dev Biol. 2010 Aug 15; 344(2):543-54. PMID: 20399765.
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McLennan R, Teddy JM, Kasemeier-Kulesa JC, Romine MH, Kulesa PM. Vascular endothelial growth factor (VEGF) regulates cranial neural crest migration in vivo. Dev Biol. 2010 Mar 01; 339(1):114-25. PMID: 20036652.
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Kulesa PM, Stark DA, Steen J, Lansford R, Kasemeier-Kulesa JC. Watching the assembly of an organ a single cell at a time using confocal multi-position photoactivation and multi-time acquisition. Organogenesis. 2009 Oct; 5(4):238-47. PMID: 20539744.
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Kulesa PM, Lefcort F, Kasemeier-Kulesa JC. The migration of autonomic precursor cells in the embryo. Auton Neurosci. 2009 Nov 17; 151(1):3-9. PMID: 19783486.
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Kasemeier-Kulesa JC, Teddy JM, Postovit LM, Seftor EA, Seftor RE, Hendrix MJ, Kulesa PM. Reprogramming multipotent tumor cells with the embryonic neural crest microenvironment. Dev Dyn. 2008 Oct; 237(10):2657-66. PMID: 18629870.
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Stark DA, Kasemeier-Kulesa JC, Kulesa PM. Photoactivation cell labeling for cell tracing in avian development. CSH Protoc. 2008 Mar 01; 2008:pdb.prot4975. PMID: 21356791.
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Kulesa PM, Teddy JM, Stark DA, Smith SE, McLennan R. Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR. Dev Biol. 2008 Apr 15; 316(2):275-87. PMID: 18328476.
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Bhattacharyya S, Kulesa PM, Fraser SE. Vital labeling of embryonic cells using fluorescent dyes and proteins. Methods Cell Biol. 2008; 87:187-210. PMID: 18485298.
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Rupp PA, Kulesa PM. A role for RhoA in the two-phase migratory pattern of post-otic neural crest cells. Dev Biol. 2007 Nov 01; 311(1):159-71. PMID: 17900555.
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Rupp PA, Kulesa PM. High-Resolution, Intravital 4D Confocal Time-Lapse Imaging in Avian Embryos Using a Teflon Culture Chamber Design. CSH Protoc. 2007 Jul 01; 2007:pdb.prot4790. PMID: 21357128.
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Kasemeier-Kulesa JC, Lefcort F, Kulesa PM. Sagittal Explant Culture for 3D Confocal Time-Lapse Analysis of Chick Peripheral Nervous System Formation. CSH Protoc. 2007 Jul 01; 2007:pdb.prot4791. PMID: 21357129.
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Kulesa PM, Kasemeier-Kulesa JC. Construction of a Heated Incubation Chamber around a Microscope Stage for Time-Lapse Imaging. CSH Protoc. 2007 Jul 01; 2007:pdb.prot4792. PMID: 21357130.
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Stark DA, Kulesa PM. An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model. Dev Dyn. 2007 Jun; 236(6):1583-94. PMID: 17486622.
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Kulesa PM, Schnell S, Rudloff S, Baker RE, Maini PK. From segment to somite: segmentation to epithelialization analyzed within quantitative frameworks. Dev Dyn. 2007 Jun; 236(6):1392-402. PMID: 17497694.
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Hendrix MJ, Seftor EA, Seftor RE, Kasemeier-Kulesa J, Kulesa PM, Postovit LM. Reprogramming metastatic tumour cells with embryonic microenvironments. Nat Rev Cancer. 2007 Apr; 7(4):246-55. PMID: 17384580.
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Kasemeier-Kulesa JC, Bradley R, Pasquale EB, Lefcort F, Kulesa PM. Eph/ephrins and N-cadherin coordinate to control the pattern of sympathetic ganglia. Development. 2006 Dec; 133(24):4839-47. PMID: 17108003.
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McLennan R, Kulesa PM. In vivo analysis reveals a critical role for neuropilin-1 in cranial neural crest cell migration in chick. Dev Biol. 2007 Jan 01; 301(1):227-39. PMID: 16959234.
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Kulesa PM, Kasemeier-Kulesa JC, Teddy JM, Margaryan NV, Seftor EA, Seftor RE, Hendrix MJ. Reprogramming metastatic melanoma cells to assume a neural crest cell-like phenotype in an embryonic microenvironment. Proc Natl Acad Sci U S A. 2006 Mar 07; 103(10):3752-7. PMID: 16505384.
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Teddy JM, Lansford R, Kulesa PM. Four-color, 4-D time-lapse confocal imaging of chick embryos. Biotechniques. 2005 Nov; 39(5):703-10. PMID: 16312219.
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Kulesa PM, Lu CC, Fraser SE. Time-lapse analysis reveals a series of events by which cranial neural crest cells reroute around physical barriers. Brain Behav Evol. 2005; 66(4):255-65. PMID: 16254414.
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Stark DA, Kulesa PM. In vivo marking of single cells in chick embryos using photoactivation of GFP. Curr Protoc Cell Biol. 2005 Oct; Chapter 12:Unit 12.8. PMID: 18228460.
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Stark DA, Kulesa PM. Photoactivatable green fluorescent protein as a single-cell marker in living embryos. Dev Dyn. 2005 Jul; 233(3):983-92. PMID: 15861406.
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Kasemeier-Kulesa JC, Kulesa PM, Lefcort F. Imaging neural crest cell dynamics during formation of dorsal root ganglia and sympathetic ganglia. Development. 2005 Jan; 132(2):235-45. PMID: 15590743.
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Teddy JM, Kulesa PM. In vivo evidence for short- and long-range cell communication in cranial neural crest cells. Development. 2004 Dec; 131(24):6141-51. PMID: 15548586.
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Kulesa PM. Developmental imaging: Insights into the avian embryo. Birth Defects Res C Embryo Today. 2004 Sep; 72(3):260-6. PMID: 15495184.
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Kulesa P, Ellies DL, Trainor PA. Comparative analysis of neural crest cell death, migration, and function during vertebrate embryogenesis. Dev Dyn. 2004 Jan; 229(1):14-29. PMID: 14699574.
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Patten I, Kulesa P, Shen MM, Fraser S, Placzek M. Distinct modes of floor plate induction in the chick embryo. Development. 2003 Oct; 130(20):4809-21. PMID: 12917296.
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Jones EA, Crotty D, Kulesa PM, Waters CW, Baron MH, Fraser SE, Dickinson ME. Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo culture. Genesis. 2002 Dec; 34(4):228-35. PMID: 12434332.
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Kulesa PM, Fraser SE. Cell dynamics during somite boundary formation revealed by time-lapse analysis. Science. 2002 Nov 01; 298(5595):991-5. PMID: 12411697.
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Mathis L, Kulesa PM, Fraser SE. FGF receptor signalling is required to maintain neural progenitors during Hensen's node progression. Nat Cell Biol. 2001 Jun; 3(6):559-66. PMID: 11389440.
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Kulesa P, Bronner-Fraser M, Fraser S. In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest. Development. 2000 Jul; 127(13):2843-52. PMID: 10851129.
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Kulesa PM, Fraser SE. In ovo time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches. Development. 2000 Mar; 127(6):1161-72. PMID: 10683170.
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Kulesa PM, Fraser SE. Confocal imaging of living cells in intact embryos. Methods Mol Biol. 1999; 122:205-22. PMID: 10231794.
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Kulesa PM, Fraser SE. Neural crest cell dynamics revealed by time-lapse video microscopy of whole embryo chick explant cultures. Dev Biol. 1998 Dec 15; 204(2):327-44. PMID: 9882474.
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Kulesa PM, Fraser SE. Segmentation of the vertebrate hindbrain: a time-lapse analysis. Int J Dev Biol. 1998; 42(3):385-92. PMID: 9654023.
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Krull CE, Kulesa PM. Embryonic explant and slice preparations for studies of cell migration and axon guidance. Curr Top Dev Biol. 1998; 36:145-59. PMID: 9342526.
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Burgess PK, Kulesa PM, Murray JD, Alvord EC. The interaction of growth rates and diffusion coefficients in a three-dimensional mathematical model of gliomas. J Neuropathol Exp Neurol. 1997 Jun; 56(6):704-13. PMID: 9184661.
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Lubkin SR, Romatowski J, Zhu M, Kulesa PM, White KA. Evaluation of feline leukemia virus control measures. J Theor Biol. 1996 Jan 07; 178(1):53-60. PMID: 8857332.
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1996 | 1 | 1997 | 1 | 1998 | 3 | 1999 | 1 | 2000 | 2 | 2001 | 1 | 2002 | 2 | 2003 | 1 | 2004 | 4 | 2005 | 4 | 2006 | 3 | 2007 | 7 | 2008 | 4 | 2009 | 3 | 2010 | 7 | 2011 | 3 | 2012 | 5 | 2013 | 4 | 2014 | 2 | 2015 | 5 | 2016 | 2 | 2017 | 3 | 2018 | 4 | 2019 | 5 | 2020 | 2 | 2021 | 4 | 2023 | 3 | 2024 | 2 |
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