Diffuse intrinsic pontine glioma (DIPG)or diffuse midline glioma (DMG) is a highly malignant tumor in the brain stem and primarily affects children. There is no cure for this tumor, and the survival rate is very low. Due to the tumor’s location, surgical resection of tumor is extremely difficult, and no effective chemotherapy drug is available. Recently, overexpression of Crk was reported in DIPG patients. In this study, patient-derived DIPG cell lines will be used to study roles of Crk and CrkL in DIPG cell growth, migration, and invasion. Genetically manipulating the expression levels of Crk and CrkL protein in DIPG cell lines and comparing the results with those obtained from GBM cells would provide novel insights into the therapeutic intervention of DIPG. Initial results suggest that Crk and CrkL play essential overlapping roles in DIPG cell migration and invasion.